GlyCosmos Portal

Submissions

calcium voltage-gated channel subunit alpha1 H

Summary

Gene Symbol

CACNA1H

Organism

Homo sapiens (human)

External Links

NCBI Gene

8912

PubChem

8912

Alliance of Genome Resources

HGNC:1395

Annotation

Keyword

3D-structure
Alternative splicing
Calcium channel
Cell membrane
Coiled coil
Disease variant
Epilepsy
Glycoprotein
Metal-binding
Proteomics identification
Reference proteome
Repeat
Transmembrane helix
Voltage-gated channel
Zinc

Proteins

Displaying **all 3** entries
UniProt	Protein Name
O95180	Low-voltage-activated calcium channel alpha1 3.2 subunit Voltage-gated calcium channel subunit alpha Cav3.2
B3KQH9
A0A1W2PQW2

Annotation information from UniProt.

Gene Ontology (GO)

Displaying entries **1 - 5** of 13 in total

1

2

3

Next ›

Last »
GO Term	Evidence Code	PMID
muscle contraction	TAS	9670923
muscle organ development	NAS	9670923
myoblast fusion	TAS	10861024
regulation of heart contraction	TAS	9670923
aldosterone biosynthetic process	IMP	19342457

GO Hierarchy

biological process

metabolic process [inference]

biosynthetic process [inference]

hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

cellular biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

ketone biosynthetic process [inference]

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

small molecule biosynthetic process [inference]

ketone biosynthetic process [inference]

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

organic substance biosynthetic process [inference]

lipid biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

ketone biosynthetic process [inference]

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

olefinic compound biosynthetic process [inference]

organic cyclic compound biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

organic hydroxy compound biosynthetic process [inference]

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

hormone metabolic process [inference]

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

cellular metabolic process [inference]

cellular aldehyde metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

cellular ketone metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

ketone biosynthetic process [inference]

cellular biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

ketone biosynthetic process [inference]

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

primary metabolic process [inference]

lipid metabolic process [inference]

steroid metabolic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

glucocorticoid metabolic process [inference]

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

lipid biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

small molecule metabolic process [inference]

alcohol metabolic process [inference]

primary alcohol metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

primary alcohol biosynthetic process [inference]

cortisol metabolic process [inference]

cortisol biosynthetic process

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

tertiary alcohol metabolic process [inference]

cortisol metabolic process [inference]

cortisol biosynthetic process

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

cellular ketone metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

ketone biosynthetic process [inference]

small molecule biosynthetic process [inference]

ketone biosynthetic process [inference]

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

organic substance metabolic process [inference]

cellular aldehyde metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

lipid metabolic process [inference]

steroid metabolic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

glucocorticoid metabolic process [inference]

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

lipid biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

cellular ketone metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

ketone biosynthetic process [inference]

olefinic compound metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

olefinic compound biosynthetic process [inference]

organic cyclic compound metabolic process [inference]

steroid metabolic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

glucocorticoid metabolic process [inference]

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

organic cyclic compound biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

organic substance biosynthetic process [inference]

lipid biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

ketone biosynthetic process [inference]

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

olefinic compound biosynthetic process [inference]

organic cyclic compound biosynthetic process [inference]

steroid biosynthetic process [inference]

steroid hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

glucocorticoid biosynthetic process [inference]

cortisol biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

organic hydroxy compound biosynthetic process [inference]

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

organic hydroxy compound metabolic process [inference]

alcohol metabolic process [inference]

primary alcohol metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

primary alcohol biosynthetic process [inference]

cortisol metabolic process [inference]

cortisol biosynthetic process

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

tertiary alcohol metabolic process [inference]

cortisol metabolic process [inference]

cortisol biosynthetic process

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

organic hydroxy compound biosynthetic process [inference]

alcohol biosynthetic process [inference]

primary alcohol biosynthetic process [inference]

tertiary alcohol biosynthetic process [inference]

cortisol biosynthetic process

cellular process [inference]

syncytium formation [inference]

syncytium formation by plasma membrane fusion [inference]

myoblast fusion

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cellular metabolic process [inference]

cellular aldehyde metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

cellular ketone metabolic process [inference]

aldosterone metabolic process [inference]

aldosterone biosynthetic process

cortisol metabolic process [inference]

cortisol biosynthetic process

ketone biosynthetic process [inference]

cellular biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

ketone biosynthetic process [inference]

aldehyde biosynthetic process [inference]

aldosterone biosynthetic process

cellular response to stimulus [inference]

cellular response to chemical stimulus [inference]

cellular response to inorganic substance [inference]

cellular response to metal ion [inference]

cellular response to potassium ion

cellular response to organic substance [inference]

cellular response to hormone stimulus

cellular response to oxygen-containing compound [inference]

cellular response to potassium ion

cellular response to salt [inference]

cellular response to potassium ion

transmembrane transport [inference]

monoatomic ion transmembrane transport [inference]

monoatomic cation transmembrane transport [inference]

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

inorganic ion transmembrane transport [inference]

inorganic cation transmembrane transport

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

inorganic ion import across plasma membrane [inference]

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

import across plasma membrane [inference]

inorganic ion import across plasma membrane [inference]

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

cell-cell fusion [inference]

syncytium formation by plasma membrane fusion [inference]

myoblast fusion

multicellular organismal process [inference]

system process [inference]

muscle system process [inference]

muscle contraction

developmental process [inference]

anatomical structure formation involved in morphogenesis [inference]

syncytium formation [inference]

syncytium formation by plasma membrane fusion [inference]

myoblast fusion

anatomical structure development [inference]

animal organ development [inference]

muscle organ development

muscle structure development [inference]

muscle organ development

response to stimulus [inference]

response to endogenous stimulus [inference]

response to hormone [inference]

cellular response to hormone stimulus

cellular response to endogenous stimulus [inference]

cellular response to hormone stimulus

response to chemical [inference]

response to organic substance [inference]

response to hormone [inference]

cellular response to hormone stimulus

cellular response to organic substance [inference]

cellular response to hormone stimulus

response to inorganic substance [inference]

response to metal ion [inference]

response to potassium ion [inference]

cellular response to potassium ion

cellular response to metal ion [inference]

cellular response to potassium ion

cellular response to inorganic substance [inference]

cellular response to metal ion [inference]

cellular response to potassium ion

cellular response to chemical stimulus [inference]

cellular response to inorganic substance [inference]

cellular response to metal ion [inference]

cellular response to potassium ion

cellular response to organic substance [inference]

cellular response to hormone stimulus

cellular response to oxygen-containing compound [inference]

cellular response to potassium ion

cellular response to salt [inference]

cellular response to potassium ion

response to oxygen-containing compound [inference]

response to potassium ion [inference]

cellular response to potassium ion

cellular response to oxygen-containing compound [inference]

cellular response to potassium ion

response to salt [inference]

response to potassium ion [inference]

cellular response to potassium ion

cellular response to salt [inference]

cellular response to potassium ion

cellular response to stimulus [inference]

cellular response to chemical stimulus [inference]

cellular response to inorganic substance [inference]

cellular response to metal ion [inference]

cellular response to potassium ion

cellular response to organic substance [inference]

cellular response to hormone stimulus

cellular response to oxygen-containing compound [inference]

cellular response to potassium ion

cellular response to salt [inference]

cellular response to potassium ion

localization [inference]

establishment of localization [inference]

transport [inference]

monoatomic ion transport [inference]

monoatomic cation transport [inference]

metal ion transport [inference]

calcium ion transport [inference]

calcium ion import

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

monoatomic cation transmembrane transport [inference]

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

monoatomic ion transmembrane transport [inference]

monoatomic cation transmembrane transport [inference]

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

transmembrane transport [inference]

monoatomic ion transmembrane transport [inference]

monoatomic cation transmembrane transport [inference]

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

inorganic ion transmembrane transport [inference]

inorganic cation transmembrane transport

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

inorganic ion import across plasma membrane [inference]

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

import across plasma membrane [inference]

inorganic ion import across plasma membrane [inference]

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

organic substance transport [inference]

calcium ion transport [inference]

calcium ion import

calcium ion transmembrane transport [inference]

calcium ion transmembrane import into cytosol [inference]

calcium ion import across plasma membrane

import into cell [inference]

inorganic ion import across plasma membrane [inference]

inorganic cation import across plasma membrane [inference]

calcium ion import across plasma membrane

biological regulation [inference]

regulation of biological process [inference]

positive regulation of biological process [inference]

positive regulation of reproductive process [inference]

positive regulation of acrosome reaction

regulation of multicellular organismal process [inference]

regulation of system process [inference]

regulation of blood circulation [inference]

regulation of heart contraction

regulation of reproductive process [inference]

regulation of acrosome reaction [inference]

positive regulation of acrosome reaction

positive regulation of reproductive process [inference]

positive regulation of acrosome reaction

regulation of biological quality [inference]

regulation of hormone levels [inference]

hormone metabolic process [inference]

C21-steroid hormone metabolic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

mineralocorticoid metabolic process [inference]

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

aldosterone metabolic process [inference]

aldosterone biosynthetic process

hormone biosynthetic process [inference]

C21-steroid hormone biosynthetic process [inference]

aldosterone biosynthetic process

mineralocorticoid biosynthetic process [inference]

aldosterone biosynthetic process

regulation of membrane potential

Displaying **all 4** entries
GO Term	Evidence Code	PMID
plasma membrane	TAS
plasma membrane	IDA	27149520
voltage-gated calcium channel complex	IBA
membrane	IDA	21084288

GO Hierarchy

cellular component

protein-containing complex [inference]

membrane protein complex [inference]

plasma membrane protein complex [inference]

voltage-gated calcium channel complex

transmembrane transporter complex [inference]

monoatomic ion channel complex [inference]

cation channel complex [inference]

calcium channel complex [inference]

voltage-gated calcium channel complex

transporter complex [inference]

transmembrane transporter complex [inference]

monoatomic ion channel complex [inference]

cation channel complex [inference]

calcium channel complex [inference]

voltage-gated calcium channel complex

cellular anatomical entity [inference]

membrane

plasma membrane

Displaying entries **1 - 5** of 8 in total

1

2

Next ›

Last »
GO Term	Evidence Code	PMID
voltage-gated monoatomic ion channel activity	IDA	27149520
voltage-gated calcium channel activity	IDA	9930755
voltage-gated calcium channel activity	IMP	24277868 25907736 27729216
protein binding	IPI	27149520
high voltage-gated calcium channel activity	IBA

GO Hierarchy

molecular function

transporter activity [inference]

transmembrane transporter activity [inference]

monoatomic ion transmembrane transporter activity [inference]

monoatomic ion channel activity [inference]

voltage-gated monoatomic ion channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

monoatomic cation channel activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

monoatomic cation transmembrane transporter activity [inference]

monoatomic cation channel activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

metal ion transmembrane transporter activity [inference]

calcium ion transmembrane transporter activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

inorganic molecular entity transmembrane transporter activity [inference]

inorganic cation transmembrane transporter activity [inference]

metal ion transmembrane transporter activity [inference]

calcium ion transmembrane transporter activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

passive transmembrane transporter activity [inference]

channel activity [inference]

monoatomic ion channel activity [inference]

voltage-gated monoatomic ion channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

monoatomic cation channel activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

gated channel activity [inference]

voltage-gated channel activity [inference]

voltage-gated monoatomic ion channel activity

voltage-gated monoatomic cation channel activity [inference]

voltage-gated calcium channel activity

salt transmembrane transporter activity [inference]

calcium ion transmembrane transporter activity [inference]

calcium channel activity [inference]

voltage-gated calcium channel activity

binding [inference]

protein binding

scaffold protein binding

ion binding [inference]

cation binding [inference]

metal ion binding

Gene Ontology information from NCBI Gene and UniProt.

OrthoDB (Group)

Group level

Eukaryota

Group Name

voltage-dependent t-type calcium channel subunit alpha

Functional Category

E: Amino acid transport and metabolism
J: Translation, ribosomal structure and biogenesis
P: Inorganic ion transport and metabolism
T: Signal transduction mechanisms

Displaying **all 8** entries
Gene Ontology
calcium ion import
calcium ion transmembrane transport
low voltage-gated calcium channel activity
membrane depolarization during action potential
monoatomic ion channel activity
monoatomic ion transport
positive regulation of calcium ion-dependent exocytosis
voltage-gated sodium channel activity

Displaying **all 4** entries
InterPro
Ion transport domain
Voltage-dependent calcium channel, T-type, alpha-1 subunit
Voltage-dependent channel domain superfamily
Voltage-gated cation channel calcium and sodium

Disease

Disease Ontology

Displaying **all 2** entries
DO ID	Disease Name	Source
DOID:1825	childhood absence epilepsy	Alliance of Genome Resources
DOID:446	primary hyperaldosteronism	Alliance of Genome Resources

Ortholog

Displaying entries **1 - 10** of 53 in total

1

2

3

4

5

6

Next ›

Last »
Species	Gene ID	Alliance of Genome Resources	Orthologous MAtrix
Drosophila melanogaster	8911	FB:FBgn0264386
Xenopus tropicalis	8912	Xenbase:XB-GENE-6042121
Drosophila melanogaster	8913	FB:FBgn0264386
Drosophila melanogaster	12291	FB:FBgn0264386
Drosophila melanogaster	56827	FB:FBgn0264386
Mus musculus	58226		MOUSE21002
Rattus norvegicus	114862	Xenbase:XB-GENE-6042121	RATNO01845
Bos taurus	282412		BOVIN19984
Canis lupus familiaris	403533		CANLF16730
Pan troglodytes	453813		PANTR11393

GlyTouCan

GlyComb

GlycoPOST

UniCarb-DR

LM-GlycoRepo

All Resources

Other Information

Tools

Search Tools

Guidelines

Summary

External Links

Annotation

OrthoDB (Group)

International Collaboration

Acknowledgements